![]() squarrosulus 55A into three spots (Rfs 0.75, 0.50, 0.17), the mycelial extracts of L. On silica gel thin-layer chromatograms, the crude extracts of Lentinus were separated into several bioautographic spots for the filtrate extracts of L. In parallel, the spots were detected by viewing with chemical revelations or under ultraviolet radiations at 254 nm or 366 nm. On these plates, the extracts were separated into several bioautographic spots or growth inhibition zones. The dry silica gel plates were then seeded with Bacillus subtilis and incubated at 35 ☌ for one night. For this purpose, the crude extracts were deposited as spots on silica gel plates and developed in a n-butanol-acetic acid-water mixture (3:1:1). ![]() The antimicrobial compounds extracted either from culture filtrates or mycelia of several tropical Lentinus species could be detected their existences and locations by bioautographic method.
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